Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (51): 9533-9540.doi: 10.3969/j.issn.2095-4344.2012.51.007

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Antimicrobial efficacy of nano-silver endodontic irrigations for an in vitro model infected byEnterococcus faecalis in starvation

Chen Jian-hong, Wang Ping, Peng Meng, Tang Qian, Wen Yun-sheng   

  • Online:2012-12-16 Published:2012-12-16

Abstract:

BACKGROUND: Effective root canal irrigation in root canal treatment can significantly reduce bacteria and their products within the root canal system, improving the treatment effectiveness and prognosis.
OBJECTIVE: To evaluate the antimicrobial effects of two kinds of nano-silver endodontic irrigates containing chlorhexidine acetate or triclosan against Entercoccus faecalis in starvation in root canals. 
METHODS: Totally 45 infected premolars models used in the experiment were divided into three groups randomly, all of which were prepared with Protaper hand instruments to F3, and were irrigated with irrigations containing chlorhexidine acetate, triclosan and NaClO. Microbial growth was analyzed by turbidity of the culture medium, and then colony-forming units were counted on the plates.
RESULTS AND CONCLUSION: There was a statistically significant difference in colony-forming units of Entercoccus faecalis before and after irrigation (P < 0.01). All of the three irrigations were antimicrobial. There was no significant difference in the antimicrobial effect between chlorhexidine acetate nano-silver and NaClO (P > 0.05). Chlorhexidine acetate nano-silver and NaClO showed a better antimicrobial effect than triclosan nano-silver, which was statistically significant (P < 0.05). These findings indicated that chlorhexidine acetate nano-silver, triclosan nano-silver and NaClO were antimicrobial, which could not eradicate Entercoccus faecalis in the root canals. Antimicrobial effects of chlorhexidine acetate nano-silver and NaClO were similar, which were better than triclosan nano-silver.